Effect of Semen Diluents and Dilution Rates on Motility of Guinea Fowl Spermatozoa Under Short-Term Storage

The assessment of spermatozoa motility is one of the most often used parameters for semen evaluation. The study was conducted at Poultry Research Station, Madhavaram Milk Colony, Chennai600 051, to  nd the effect of different semen diluents and dilution ratio on per cent spermatozoa motility of pooled guinea fowl semen at 5o C under short term storage(0, 3 and 6 Hours). Irrespective of the storage periods and dilution ratio, the semen diluted with BPSE had shown better spermatozoa motility than with IMV diluent. Superior motility at the maximum storage period of six hours was found in semen diluted with Beltsville Poultry Semen Extender at the ratio of 1:2 (77.25±1.32%). Based on the study, it is concluded that dilution ratio and storage period had signi cant in uence on spermatozoa motility and the same must be considered for better fertility by Arti cial insemination (AI) in guinea fowls.